874374-25-1
874374-25-1 structure
- Name: Ned 19
- Chemical Name: 1-(3-{[4-(2-Fluorophenyl)-1-piperazinyl]methyl}-4-methoxyphenyl)- 2,3,4,9-tetrahydro-1H-β-carboline-3-carboxylic acid
- CAS Number: 874374-25-1
- Molecular Formula: C30H31FN4O3
- Molecular Weight: 514.59100
- Catalog: Signaling Pathways Membrane Transporter/Ion Channel Calcium Channel
- Create Date: 2016-01-31 08:49:47
- Modify Date: 2024-01-02 10:15:55
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Ned 19 is a selective membrane-permeant non competitive NAADP antagonist and inhibits NAADP-mediated Ca2+ signaling, with an IC50 of 65 nM[1]. Ned 19 strongly inhibits tumor growth and vascularization as well as lung metastases in mice[2].
| Name | 1-(3-{[4-(2-Fluorophenyl)-1-piperazinyl]methyl}-4-methoxyphenyl)- 2,3,4,9-tetrahydro-1H-β-carboline-3-carboxylic acid |
|---|
| Description | Ned 19 is a selective membrane-permeant non competitive NAADP antagonist and inhibits NAADP-mediated Ca2+ signaling, with an IC50 of 65 nM[1]. Ned 19 strongly inhibits tumor growth and vascularization as well as lung metastases in mice[2]. |
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| Related Catalog | |
| Target |
Ca2+ |
| In Vitro | Ned 19 (25-100 μM; 24-72 hours) reduces cell proliferation[2]. Ned 19 (25-100 μM; 24-72 hours) reduces markedly the cell number[2]. Ned 19 (25-100 μM; 24-72 hours) reduces the S phase percentage and increases of the G0/G1 phase percentage evaluated by cell cycle analysis[2]. Ned 19 (25-100 μM; 24-72 hours) induces cell apoptosis a time-dependent manner[2]. Ned 19 (25-100 μM; 24-72 hours) reduces expression of N-cadherin and increases expression of E-cadherin, affecting the cell migratory behavior[2]. Cell Proliferation Assay[2] Cell Line: B16 cells Concentration: 25, 50, 100 μM Incubation Time: 24, 48, 72 hours Result: Reduced cell proliferation. Cell Viability Assay[2] Cell Line: B16 cells Concentration: 25, 50, 100 μM Incubation Time: 24, 48, 72 hours Result: Reduced markedly the cell number. Cell Cycle Analysis[2] Cell Line: B16 cells Concentration: 25, 50, 100 μM Incubation Time: 24, 48, 72 hours Result: Reduced the S phase percentage and increased of the G0/G1 phase percentage. Apoptosis Analysis[2] Cell Line: B16 cells Concentration: 25, 50, 100 μM Incubation Time: 24, 48, 72 hours Result: Induced cell apoptosis a time-dependent manner. Western Blot Analysis[2] Cell Line: B16 cells Concentration: 25, 50, 100 μM Incubation Time: 24, 48, 72 hours Result: Reduced expression of N-cadherin and increased expression of E-cadherin. |
| In Vivo | Ned 19 (i.p.; 5 mg/Kg/every second day; 4 week) impaires severely tumor growth[2]. Animal Model: Adult male C57BL/6 mice[2] Dosage: 5 mg/Kg Administration: I.p.; every second day; 4 week Result: Impaired severely tumor growth. |
| References |
| Molecular Formula | C30H31FN4O3 |
|---|---|
| Molecular Weight | 514.59100 |
| Exact Mass | 514.23800 |
| PSA | 80.83000 |
| LogP | 4.65780 |