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2321-07-5

2321-07-5 structure
2321-07-5 structure
Name fluorescein
Synonyms D and C Yellow No. 7
D&C Yellow No. 7
D+C Yellow No. 7
Fluorescein (free acid)
3',6'-Dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
3',6'-Fluorandiol
Fluorecein
Eosin
YELLOW 7
3',6'-Dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one
KI201
MFCD00005050
Acid Yellow 73
3',6'-Dihydroxy-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one
Fluorescite
EINECS 219-031-8
Uranine Yellow
C.I. Acid Yellow 73
angiofluor
Soluble fluorescein
D & C Yellow no. 7
Fluor-I-Strip
D & C Yellow no. 8
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-
Fluorescein
Ful-Glo
Funduscein
Description Fluorescein is a synthetic fluorescent tracer.
Related Catalog
In Vitro The fluorescein dye is a common fluorescent probe. Its very high molar absorptivity at the wavelength of the argon laser (488 nm), large fluorescence quantum yield and high photostability makes it a very useful and sensitive fluorescent label. Fluorescein is commercially available in many derivatives, such as fluorescein isothiocyanate and fluorescein succinimidyl ester, that can be covalently attached to macromolecules and to amino acids. The labeled molecules can be detected with very high sensitivity which is utilized in, for example, capillary electrophoresis. The emission spectrum of fluorescein overlaps extensively the absorption spectrum of tetramethyl rhodamine, which is a related strongly fluorescent dye, making this pair very suitable for energy transfer experiments to determine distances within and between labeled macromolecules. Fluorescein in aqueous solution occurs in cationic, neutral, anionic and dianionic forms making its absorption and fluoresence properties strongly pH dependent[1].
References

[1]. R Sjöback, et al. Absorption and fluorescence properties of fluorescein. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Density 1.6±0.1 g/cm3
Boiling Point 620.8±55.0 °C at 760 mmHg
Melting Point 320 °C(lit.)
Molecular Formula C20H12O5
Molecular Weight 332.306
Flash Point 232.6±25.0 °C
Exact Mass 332.068481
PSA 87.74000
LogP 2.98
Vapour Pressure 0.0±1.9 mmHg at 25°C
Index of Refraction 1.792
Storage condition −20°C
Water Solubility insoluble

CHEMICAL IDENTIFICATION

RTECS NUMBER :
LM5075000
CHEMICAL NAME :
Fluorescein
CAS REGISTRY NUMBER :
2321-07-5
BEILSTEIN REFERENCE NO. :
0094324
LAST UPDATED :
199707
DATA ITEMS CITED :
10
MOLECULAR FORMULA :
C20-H12-O5
MOLECULAR WEIGHT :
332.32
WISWESSER LINE NOTATION :
T C666 BO EVJ IR BVQ& MQ

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Intraperitoneal
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
600 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
300 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Unreported
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
600 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Rodent - rabbit
DOSE/DURATION :
300 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Unreported
SPECIES OBSERVED :
Rodent - guinea pig
DOSE/DURATION :
400 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value

MUTATION DATA

TYPE OF TEST :
DNA adduct
TEST SYSTEM :
Bacteria - Escherichia coli
DOSE/DURATION :
15 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 89,95,1981 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - 33350 No. of Facilities: 495 (estimated) No. of Industries: 18 No. of Occupations: 21 No. of Employees: 5188 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - 33350 No. of Facilities: 2171 (estimated) No. of Industries: 42 No. of Occupations: 55 No. of Employees: 88495 (estimated) No. of Female Employees: 21558 (estimated)
Symbol GHS07
GHS07
Signal Word Warning
Hazard Statements H319
Precautionary Statements P305 + P351 + P338
Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes Xi:Irritant;
Risk Phrases R36;R43
Safety Phrases S22-S24/25-S37/39-S26
RIDADR NONH for all modes of transport
WGK Germany 3
RTECS LM5075000
Packaging Group I; II; III
HS Code 3204200000
HS Code 2932999099
Summary 2932999099. other heterocyclic compounds with oxygen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

Chemsrc provides CAS#:2321-07-5 MSDS, density, melting point, boiling point, structure, formula, molecular weight, synthetic route, etc.
title: CAS No. 2321-07-5 | Chemsrc address: https://www.chemsrc.com/en/baike/1186873.html

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