149402-51-7
149402-51-7 structure
- Name: HPPH
- Chemical Name: Photochlor
- CAS Number: 149402-51-7
- Molecular Formula: C39H48N4O4
- Molecular Weight: 636.823
- Catalog: Research Areas Cancer
- Create Date: 2018-09-01 11:48:58
- Modify Date: 2024-01-02 12:47:40
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HPPH is a second generation photosensitizer, which acts as a photodynamic therapy (PDT) agent.
| Name | Photochlor |
|---|---|
| Synonyms |
2-(1-Hexyloxyethyl)-2-devinyl pyropheophorbide-a
3-{(3S,4S)-14-Ethyl-9-[1-(hexyloxy)ethyl]-4,8,13,18-tetramethyl-20-oxo-24,25-dihydro-3-phorbinyl}propanoic acid 2-[1'-(Hexyloxy)ethyl]-2-devinylpyropheophorbide A 3-Devinyl-3-(1'-hexyloxy)ethylpyropheophorbide A 3-Phorbinepropanoic acid, 14-ethyl-9-[1-(hexyloxy)ethyl]-24,25-dihydro-4,8,13,18-tetramethyl-20-oxo-, (3S,4S)- (3S,4S)-14-Ethyl-9-[1-(hexyloxy)ethyl]-4,8,13,18-tetramethyl-20-oxo-3-phorbinepropanoic acid HPPH |
| Description | HPPH is a second generation photosensitizer, which acts as a photodynamic therapy (PDT) agent. |
|---|---|
| Related Catalog | |
| In Vitro | Fluorescence image of 4T1 cells incubated with 0.49 µg/mL GO-PEG, 1 μM HPPH (free HPPH) or equivalent amount of GO-PEG-HPPH (1 µM HPPH and 0.49 µg/mL GO-PEG) after 24 h. The cellular uptake of GO-PEG-HPPH and HPPH is investigated with 4T1 murine mammary cancer cells. The cells are incubated with GO-PEG-HPPH and free HPPH at equivalent HPPH concentration (1 µM) for 24 h and then observed with a confocal microscope. Cells treated with GO-PEG-HHPH shows stronger fluorescence signal than those treated with free HPPH. In fact, the fluorescence of HPPH is rather weak[1]. |
| In Vivo | Tumors are treated with an immune-enhancing PDT regimen followed by a tumor-controlling PDT regimen can leads to enhancement of anti-tumor immunity, while retaining effective control of primary tumor growth. To test this hypothesis, a combination treatment regimen is devised in which Colo26-HA tumor-bearing BALB/c mice are treated with a HPPH-PDT regimen known to lead to enhanced anti-tumor immunity (0.4 μmoles/kg HPPH followed 18 h later by illumination with 665 nm light for a total dose of 48 J/cm2). Following illumination, mice are rested for 9 days; on the ninth day, mice are injected with HPPH. On day 10 following the first treatment, tumors are treated with a tumor control treatment regimen (illumination with 665 nm light for a total dose of 132 J/cm2 given)[2]. |
| Cell Assay | 4T1 cells are cultured in 96-well cell culture plates at 1×104/well for 24 h and then treated with GO-PEG-HPPH, HPPH, or GO-PEG at a series of concentrations (0.078125, 0.15625, 0.3125, 0.625, 1.25, 2.5, 5, 10, and 20 μM). Then, 20 µL of MTT solution (5.0 mg/mL) is added to each well. After the 4 h incubation with the MTT, the media are removed and 100 µL of DMSO is added to solubilize the formazan crystals. The cell toxicity efficacy is measured with a microplate reader at an absorbance of 570 nm[1]. |
| Animal Admin | Mice[2] Tumor-bearing mice are injected in the tail vein with 0.4 μmol/kg HPPH or 5 mg/kg Porfimer sodium (PII), followed 18-24 h later by illumination to a total light dose of 48 J/cm2 or 132 J/cm2 delivered at a light dose-rate of 14 mW/cm2. Control mice are treated with photosensitizer or light alone. Mice receiving a combination PDT regimen are treated initially with 0.4 μmol/kg HPPH or 5 mg/kg PII followed 18-24 h later by light dose of 48 J/cm2 given at 14 mW/cm2; 9 days later, mice are again injected with photosensitizer and tumors are illuminated with light at a dose of 132 J/cm2 given at 14 mW/cm2[2]. |
| References |
| Density | 1.2±0.1 g/cm3 |
|---|---|
| Boiling Point | 980.8±65.0 °C at 760 mmHg |
| Molecular Formula | C39H48N4O4 |
| Molecular Weight | 636.823 |
| Flash Point | 547.0±34.3 °C |
| Exact Mass | 636.367554 |
| PSA | 119.57000 |
| LogP | 8.75 |
| Vapour Pressure | 0.0±0.3 mmHg at 25°C |
| Index of Refraction | 1.620 |
| Storage condition | 2-8℃ |