929895-45-4
929895-45-4 structure
- Name: Phen-DC3 Trifluoromethanesulfonate
- Chemical Name: Phen-DC3 Trifluoromethanesulfonate
- CAS Number: 929895-45-4
- Molecular Formula: C36H26F6N6O8S2
- Molecular Weight: 848.75
- Catalog: Signaling Pathways Cell Cycle/DNA Damage G-quadruplex
- Create Date: 2018-01-21 00:17:17
- Modify Date: 2024-01-08 07:21:28
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Phen-DC3 Trifluoromethanesulfonate is a G-quadruplex (G4) specific ligand which can inhibit FANCJ and DinG helicases with IC50s of 65±6 and 50±10 nM, respectively.
| Name | Phen-DC3 Trifluoromethanesulfonate |
|---|
| Description | Phen-DC3 Trifluoromethanesulfonate is a G-quadruplex (G4) specific ligand which can inhibit FANCJ and DinG helicases with IC50s of 65±6 and 50±10 nM, respectively. |
|---|---|
| Related Catalog | |
| Target |
IC50: 65±6 nM (G4 substrate, FANCJ helicase), 50±10 nM (G4 substrate, DinG helicases)[1] |
| In Vitro | In WT cells, a CEB1-WT array is rather stable but undergoes frequent rearrangements upon addition of 10 μM Phen-DC3 Trifluoromethanesulfonate (Phen-DC3). It is found that the c-Myc allele exhibits significant destabilization upon Phen-DC3 Trifluoromethanesulfonate treatment and PIF1 deletion. The CEB25-L111(T) array is stable in WT cells, it becomes unstable upon addition of Phen-DC3 Trifluoromethanesulfonate or deletion of PIF1. It is also highly destabilized in the presence of Phen-DC3 Trifluoromethanesulfonate or in the absence of PIF1. The CEB1-loop CEB25 allele remaines fully stable in both PIF1-treated and WT cells[2]. |
| Cell Assay | Briefly, untreated WT cells and pif1Δ cells from a fresh patch of cells get from a single colony bearing the parental allele size are diluted in 5 mL of YPD (2×105 cells/mL), grown for 8 generations at 30°C with shaking, and spreaded as single colony on YPD plates. To measure minisatellite instability upon Phen-DC3 Trifluoromethanesulfonate treatment, WT cells from a fresh patch on YPD are grown for 8 generations at 30°C in liquid SC containing Phen-DC3 Trifluoromethanesulfonate at 10 μM. Isolated colonies or pools of colonies are analyzed by Southern blot using the EcoRI digestion that cuts at each side of the minisatellite[2]. |
| References |
| Molecular Formula | C36H26F6N6O8S2 |
|---|---|
| Molecular Weight | 848.75 |
| Storage condition | 2-8℃ |