92285-87-5

92285-87-5 structure

Name: L82-G17
Chemical Name: 4-Chloro-5-[(2Z)-2-(3-hydroxybenzylidene)hydrazino]-3(2H)-pyridazinone
CAS Number: 92285-87-5
Molecular Formula: C₁₁H₉ClN₄O₂
Molecular Weight: 264.668
Catalog: Signaling Pathways Cell Cycle/DNA Damage DNA/RNA Synthesis
Create Date: 2021-05-30 15:33:10
Modify Date: 2024-01-12 18:10:42
L82-G17 is an uncompetitive DNA ligase I (Lig I)-selective inhibitor. L82-G17 inhibits the third step of the ligation reaction, phosphodiester bond formation. L82-G17can be used as a probe of the catalytic activity[1].

Name	4-Chloro-5-[(2Z)-2-(3-hydroxybenzylidene)hydrazino]-3(2H)-pyridazinone
Synonyms	4-Chloro-5-[(2Z)-2-(3-hydroxybenzylidene)hydrazino]-3(2H)-pyridazinone Benzaldehyde, 3-hydroxy-, 1-[2-(5-chloro-1,6-dihydro-6-oxo-4-pyridazinyl)hydrazone] 4-Chloro-5-[(2Z)-2-(3-hydroxybenzylidene)hydrazino]pyridazin-3(2H)-one

Description	L82-G17 is an uncompetitive DNA ligase I (Lig I)-selective inhibitor. L82-G17 inhibits the third step of the ligation reaction, phosphodiester bond formation. L82-G17can be used as a probe of the catalytic activity[1].
Related Catalog	Research Areas >> Cancer Signaling Pathways >> Cell Cycle/DNA Damage >> DNA/RNA Synthesis
In Vitro	L82-G17 (200 μM, 30 min) has selective uncompetitive inhibitory effect for LigI[1]. L82-G17 (0-100 μM) increases LigI binding to non-ligatable nicked DNA binding[1]. L82-G17 inhibits step 3 of the ligation reaction, phosphodiester bond formation[1]. L82-G17 (0-100 μM) inhibits DNA synthesis, cell viability and s induces DNA damage[1]. Cell Viability Assay[1] Cell Line: HeLa cells Concentration: 0-30 μM Incubation Time: 5 days Result: Reduced cell number by about 70% at 20 μM. Cell Proliferation Assay[1] Cell Line: CH12F3 WT and CH12F3Δ/Δ cells Concentration: 0-100 μM Incubation Time: 72 h Result: Had great effect on the proliferation and survival of the parental CH12F3 cells.