2182601-72-3
2182601-72-3 structure
- Name: Cy5 DBCO chloride
- Chemical Name: Cy5 DBCO chloride
- CAS Number: 2182601-72-3
- Molecular Formula: C50H53ClN4O2
- Molecular Weight: 777.43
- Catalog: Research Areas Others
- Create Date: 2022-10-31 16:52:29
- Modify Date: 2024-04-06 21:44:37
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Cy5 DBCO chloride is an azide reaction probe and the addition of DBCO molecules allows the imaging of azide-labelled biomolecules by a copper-free “Click Chemistry” reaction[1].
| Name | Cy5 DBCO chloride |
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| Description | Cy5 DBCO chloride is an azide reaction probe and the addition of DBCO molecules allows the imaging of azide-labelled biomolecules by a copper-free “Click Chemistry” reaction[1]. |
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| Related Catalog | |
| In Vitro | Cy5 DBCO chloride (2-50 μM) can control the extent of labelling by varying the concentration of DBCO-Cy5 in a dose-dependent manner, with the amount of DBCO-Cy5 bound at the cell surface increasing with increasing DBCO-Cy5 concentration[1]. Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1.Incubate the cells at a density of 3×104 cells in a cell culture dish for 3 days and then wash the cells twice with DPBS (pH 7.4). Incubate the cells according to your normal protocol. 2.Incubate the cells with Cy5 DBCO chloride at a final concentration of 20 µM for 1 h at 37°C. 3.After incubation, wash cells with DPBS (pH 7.4) and fix with formaldehyde-glutaraldehyde combination fixative for 15 min at room temperature. 4.After fixation, wash cells twice with DPBS (pH 7.4). Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader. |
| References |
| Molecular Formula | C50H53ClN4O2 |
|---|---|
| Molecular Weight | 777.43 |