863971-24-8
863971-24-8 structure
- Name: Mc-MMAE
- Chemical Name: Maleimidocaproyl-monomethylauristatin E
- CAS Number: 863971-24-8
- Molecular Formula: C49H78N6O10
- Molecular Weight: 911.17800
- Catalog: Signaling Pathways Antibody-drug Conjugate Drug-Linker Conjugates for ADC
- Create Date: 2016-07-20 01:10:44
- Modify Date: 2024-01-08 08:34:22
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Mc-MMAE is a protective group (maleimidocaproyl)-conjugated monomethyl auristatin E (MMAE), which is a potent tubulin inhibitor, is a toxin payload in antibody drug conjugate (ADC).
| Name | Maleimidocaproyl-monomethylauristatin E |
|---|---|
| Synonyms | Mc-MMAE |
| Description | Mc-MMAE is a protective group (maleimidocaproyl)-conjugated monomethyl auristatin E (MMAE), which is a potent tubulin inhibitor, is a toxin payload in antibody drug conjugate (ADC). |
|---|---|
| Related Catalog | |
| Target |
Tubulin, Antibody-drug conjugates (ADC)[1] |
| In Vitro | Synthesis of maleimidocaproyl-MMAE (mc-MMAE) requires the addition of maleimidocaproic acid to a solution of MMAE in dichloromethane followed by the addition of diethyl cyanophosphonate and diisopropylethylamine[1]. |
| Kinase Assay | Horseradish peroxidase (HRP) is thiolated with 2-iminothiolane and conjugated to mc-MMAE to generate the HRP-MMAE reporter enzyme-drug conjugate. Briefly, a thiolation reaction mixture containing 0.2 mM HRP (8 mg/mL) and 50 mM 2-iminothiolane in 25 mM sodium borate decahydrate (Na2B4O7•10H2O) buffer (pH 9.0) is incubated for 1 hour at 37°C. Unreacted 2-iminothiolane is removed by passage through a PD-10 desalting column equilibrated in PBS (pH 7.4). Peak fractions are pooled and mc-MMAE is coupled to thiolated HRP (HRP-SH) at a molar ratio of 3:1. The final conjugation reaction mixture contained 80 μM HRP-SH (3.2 mg/mL) in sodium borate buffer [50 mM H3BO3, 50 mM NaCl (pH 8.0); 80% v/v] and 240 μM mc-MMAE in ice-cold CH3CN (20% v/v). After 30 minutes on ice, the reaction is terminated with a 20-fold molar excess of free cysteine (4.8 mM) before PD-10 chromatography. Peak fractions containing HRP-MMAE (exchanged into PBS) are pooled and evaluated for extent of modification using the thiol-reactive dye, Alexa Fluor 594 C5 maleimide[1]. |
| References |
| Density | 1.144±0.06 g/cm3(Predicted) |
|---|---|
| Boiling Point | 1025.2±65.0 °C(Predicted) |
| Molecular Formula | C49H78N6O10 |
| Molecular Weight | 911.17800 |
| Exact Mass | 910.57800 |
| PSA | 195.20000 |
| LogP | 4.91180 |
| Storage condition | 2-8°C |