17817-20-8
17817-20-8 structure
- Name: Fluorescein di(β-D-galactopyranoside)
- Chemical Name: Fluorescein di(β-D-galactopyranoside)
- CAS Number: 17817-20-8
- Molecular Formula: C32H32O15
- Molecular Weight: 656.58700
- Catalog: Biochemical Carbohydrate Monosaccharide
- Create Date: 2019-01-01 22:13:05
- Modify Date: 2024-01-05 15:22:09
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Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λex=485 nm, λem=535 nm).
| Name | Fluorescein di(β-D-galactopyranoside) |
|---|---|
| Synonyms | 3',6'-bis[[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy]spiro[2-benzofuran-3,9'-xanthene]-1-one |
| Description | Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λex=485 nm, λem=535 nm). |
|---|---|
| Related Catalog | |
| In Vitro | The fluorescence produced by Fluorescein di(β-D-galactopyranoside) increases in a time- and dose-dependent manner. The level of fluorescence produced by the double-substrate method is much lower than that by the Fluorescein di(β-D-galactopyranoside) method. Results show that the fluorescein produced by Fluorescein di(β-D-galactopyranoside) in Hs68 cells is proportional to the number of passages[1]. |
| Cell Assay | The cells (5×103 cells per well) are cultured in a 96-well plate overnight for attachment, washed, and then fixed in solutions. An aliquot (100 μL) of the reaction buffer (i.e., the staining solution without X-Gal) is added into each well. Then, 10 μL of 2 mM Fluorescein di(β-D-galactopyranoside) is added per well and the plate is incubated in the dark at 37°C for 24 h without CO2 supply. After incubation at 37°C for 24 h, 100 μL of the supernatant is transferred to a 96-well plate for fluorescent measurement in triplicates. The fluorescein fluorescence is measured using a fluorometer with an excitation at 485 nm and an emission at 535 nm[1]. |
| References |
| Density | 1.74 g/cm3 |
|---|---|
| Boiling Point | 974.8ºC at 760 mmHg |
| Melting Point | 200-203ºC (dec.) |
| Molecular Formula | C32H32O15 |
| Molecular Weight | 656.58700 |
| Flash Point | 316.5ºC |
| Exact Mass | 656.17400 |
| PSA | 234.29000 |
| Vapour Pressure | 0mmHg at 25°C |
| Index of Refraction | 1.764 |
| Storage condition | −20°C |
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| Safety Phrases | 22-24/25 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| HS Code | 32129000 |