Fluorescein di(β-D-galactopyranoside)
Fluorescein di(β-D-galactopyranoside) structure
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Common Name | Fluorescein di(β-D-galactopyranoside) | ||
|---|---|---|---|---|
| CAS Number | 17817-20-8 | Molecular Weight | 656.58700 | |
| Density | 1.74 g/cm3 | Boiling Point | 974.8ºC at 760 mmHg | |
| Molecular Formula | C32H32O15 | Melting Point | 200-203ºC (dec.) | |
| MSDS | Chinese USA | Flash Point | 316.5ºC | |
Use of Fluorescein di(β-D-galactopyranoside)Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λex=485 nm, λem=535 nm). |
| Name | Fluorescein di(β-D-galactopyranoside) |
|---|---|
| Synonym | More Synonyms |
| Description | Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λex=485 nm, λem=535 nm). |
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| Related Catalog | |
| In Vitro | The fluorescence produced by Fluorescein di(β-D-galactopyranoside) increases in a time- and dose-dependent manner. The level of fluorescence produced by the double-substrate method is much lower than that by the Fluorescein di(β-D-galactopyranoside) method. Results show that the fluorescein produced by Fluorescein di(β-D-galactopyranoside) in Hs68 cells is proportional to the number of passages[1]. |
| Cell Assay | The cells (5×103 cells per well) are cultured in a 96-well plate overnight for attachment, washed, and then fixed in solutions. An aliquot (100 μL) of the reaction buffer (i.e., the staining solution without X-Gal) is added into each well. Then, 10 μL of 2 mM Fluorescein di(β-D-galactopyranoside) is added per well and the plate is incubated in the dark at 37°C for 24 h without CO2 supply. After incubation at 37°C for 24 h, 100 μL of the supernatant is transferred to a 96-well plate for fluorescent measurement in triplicates. The fluorescein fluorescence is measured using a fluorometer with an excitation at 485 nm and an emission at 535 nm[1]. |
| References |
| Density | 1.74 g/cm3 |
|---|---|
| Boiling Point | 974.8ºC at 760 mmHg |
| Melting Point | 200-203ºC (dec.) |
| Molecular Formula | C32H32O15 |
| Molecular Weight | 656.58700 |
| Flash Point | 316.5ºC |
| Exact Mass | 656.17400 |
| PSA | 234.29000 |
| Vapour Pressure | 0mmHg at 25°C |
| Index of Refraction | 1.764 |
| Storage condition | −20°C |
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| Safety Phrases | 22-24/25 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| HS Code | 32129000 |
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