442644-28-2

442644-28-2 structure

Name: JW 67
Chemical Name: Trispiro[indole-3,2'-[1,3]dioxane-5',5''-[1,3]dioxane-2'',3'''-in dole]-2,2'''(1H,1'''H)-dione
CAS Number: 442644-28-2
Molecular Formula: C₂₁H₁₈N₂O₆
Molecular Weight: 394.37700
Catalog: Signaling Pathways Stem Cell/Wnt Wnt
Create Date: 2016-07-21 22:56:57
Modify Date: 2024-01-08 13:15:48
JW67 inhibits the canonical Wnt signaling with an IC50 of 1.17μM[1]. JW67 affects the multiprotein complex consisting of β-catenin/GSK-3β/AXIN/APC/CK1 that rapidly reduces active β-catenin with a subsequent downregulation of Wnt target genes. JW67 also inhibits colorectal cancer cell growth[1].

Name	Trispiro[indole-3,2'-[1,3]dioxane-5',5''-[1,3]dioxane-2'',3'''-in dole]-2,2'''(1H,1'''H)-dione

Description	JW67 inhibits the canonical Wnt signaling with an IC50 of 1.17μM[1]. JW67 affects the multiprotein complex consisting of β-catenin/GSK-3β/AXIN/APC/CK1 that rapidly reduces active β-catenin with a subsequent downregulation of Wnt target genes. JW67 also inhibits colorectal cancer cell growth[1].
Related Catalog	Research Areas >> Cancer Signaling Pathways >> Stem Cell/Wnt >> Wnt
Target	IC50: 1.17μM (Wnt signaling)[1]
In Vitro	JW67 (1 μM; 24 hours) increases concentration of AXIN2 and decreases the active form of β-catenin[1]. JW67 affects the expression of Wnt target genes and reduces cell growth of colon cancer cell lines[1]. JW67 shows a concentrationdependent reduction in proliferation with a GI50 value of 7.8 μM[1]. Western Blot Analysis[1] Cell Line: SW480 cells Concentration: 1 μM Incubation Time: 24 hours Result: Increased the AXIN2 expression. RT-PCR[1] Cell Line: SW480 cells Concentration: 10 or 25μM Incubation Time: 72 hours Result: Reduced growth of SW480 CRC cells in vitro by inhibiting cell-cycle progression at the G1/S.
References	[1]. Waaler J, et al. Novel synthetic antagonists of canonical Wnt signaling inhibit colorectal cancer cell growth. Cancer Res. 2011;71(1):197-205.