FM4-64

Modify Date: 2024-01-02 13:45:10

FM4-64 structure	Common Name	FM4-64
	CAS Number	162112-35-8	Molecular Weight	607.50600
	Density	N/A	Boiling Point	N/A
	Molecular Formula	C₃₀H₄₅Br₂N₃	Melting Point	N/A
	MSDS	USA	Flash Point	N/A

Use of FM4-64

FM4-64, a membrane-selective red fluorescent dye, belongs to the FM family of styrylpyridinium dyes. FM4-64 is widely used to study endocytosis and exocytosis, vesicle trafficking and organelle organization in living animal[1][2].

Name	3-[4-[(1E,3E,5E)-6-[4-(diethylamino)phenyl]hexa-1,3,5-trienyl]pyridin-1-ium-1-yl]propyl-triethylazanium,dibromide
Synonym	More Synonyms

Description	FM4-64, a membrane-selective red fluorescent dye, belongs to the FM family of styrylpyridinium dyes. FM4-64 is widely used to study endocytosis and exocytosis, vesicle trafficking and organelle organization in living animal[1][2].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	FM4-64 readily translocates to the nuclear envelope (NE) at physiological temperature where it exhibits enhanced fluorescence when excited at 620-650 nm in contrast to 480-520 nm excitation in the endocytic pathway and in the endoplasmic reticulum (ER). In vitro data indicated that the dye reveals an enrichment of negatively charged lipids, presumably due to local phospholipid synthesis. Dual-excitation imaging of FM4-64 in relation to lamina-associated polypeptide-1-green fluorescent protein during mitosis suggested that the disassembly of NE preserves microscale lipid complexes in the ER[1]. For studies onplant and fungal cells, FM4-64 is usually preferred to FM1-43 due to its superior brightness, greater contrast, higher photostability, and its red-shifted emission facilitating concomitantuse with the green fluorescent protein (GFP).[3]
References	[1]. Zal T, et al. Spectral shift of fluorescent dye FM4-64 reveals distinct microenvironment of nuclear envelope in living cells. Traffic. 2006;7(12):1607-1613. [2]. Stenovec M, et al. Distinct labelling of fusion events in rat lactotrophs by FM 1-43 and FM 4-64 is associated with conformational differences. Acta Physiol (Oxf). 2007;191(1):35-42. [3]. Bolte S, et al, Satiat-Jeunemaitre B. FM-dyes as experimental probes for dissecting vesicle trafficking in living plant cells. J Microsc. 2004;214(Pt 2):159-173.

Molecular Formula	C₃₀H₄₅Br₂N₃
Molecular Weight	607.50600
Exact Mass	605.19800
PSA	7.12000
LogP	0.37770
Storage condition	-20°C

FM4-64 MSDS(Chinese)

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Safety Phrases	22-24/25
RIDADR	NONH for all modes of transport

A novel fluorescence-activated cell sorter-based screen for yeast endocytosis mutants identifies a yeast homologue of mammalian eps15.

J. Cell Biol. 135 , 1485, (1996)

A complete understanding of the molecular mechanisms of endocytosis requires the discovery and characterization of the protein machinery that mediates this aspect of membrane trafficking. A novel gene...

A cell-free assay allows reconstitution of Vps33p-dependent transport to the yeast vacuole/lysosome.

J. Cell Biol. 146 , 85, (1999)

We report a cell-free system that measures transport-coupled maturation of carboxypeptidase Y (CPY). Yeast spheroplasts are lysed by extrusion through polycarbonate filters. After differential centrif...

Pyridinium,4-[6-[4-(diethylamino)phenyl]-1,3,5-hexatrienyl]-1-[3-(triethylammonio) propyl]-,dibromide

N-(3-Triethylammoniopropyl)-4-(6-(4-(diethylamino)phenyl) hexatrienyl)pyridinium dibromide

3-[4-[(1E,3E,5E)-6-[4-(diethylamino)phenyl]hexa-1,3,5-trienyl]pyridin-1-ium-1-yl]propyl-triethylazanium dibromide

SynaptoRed™ C2