ONO-7475

Modify Date: 2024-02-05 11:54:39

ONO-7475 structure	Common Name	ONO-7475
	CAS Number	1646839-59-9	Molecular Weight	562.57
	Density	N/A	Boiling Point	N/A
	Molecular Formula	C₃₂H₂₆N₄O₆	Melting Point	N/A
	MSDS	N/A	Flash Point	N/A

Use of ONO-7475

ONO-7475 is a potent, selective, and orally active novel Axl/Mer inhibitor with IC50 values of 0.7 nM and 1.0 nM, respectively. ONO-7475 sensitizes AXL-overexpressing EGFR-mutant NSCLC cells to the EGFR-TKIs, suppresses the emergence and maintenance of tolerant cells. ONO-7475 combines with Osimertinib (HY-15772) provides a bright promise for the study of EGFR-mutated non-small cell lung cancer (NSCLC)[1].

Name	ONO-7475

Description	ONO-7475 is a potent, selective, and orally active novel Axl/Mer inhibitor with IC50 values of 0.7 nM and 1.0 nM, respectively. ONO-7475 sensitizes AXL-overexpressing EGFR-mutant NSCLC cells to the EGFR-TKIs, suppresses the emergence and maintenance of tolerant cells. ONO-7475 combines with Osimertinib (HY-15772) provides a bright promise for the study of EGFR-mutated non-small cell lung cancer (NSCLC)[1].
Related Catalog	Research Areas >> Cancer Signaling Pathways >> Protein Tyrosine Kinase/RTK >> TAM Receptor Signaling Pathways >> Protein Tyrosine Kinase/RTK >> Trk Receptor
Target	IC50: 0.7 nM (AXL)[1] IC50: 1.0 nM (MER tyrosine kinase)[2]
In Vitro	ONO-7475 is against recombinant human AXL with IC50 values of 0.414 nM and 0.7 nM in off-chip MSA and ACD cell-based tyrosine kinase assay, respectively. It is against AXL, MER, TYRO3, TRKB, PDGFR alpha, TRKA, and FLT3 activities with IC50 values of 0.7 nM, 1.0 nM, 8.7 nM, 15.8 nM, 28.9 nM, 35.7 nM and 147 nM, respectively in a Cell-based Tyrosine Kinase assay[2]. ONO-7475 (0.0001 μM-1 μM; 72 hours) increases the sensitivity to Osimertinib and Dacomitinib and reduces the viability of high AXL-expressing PC-9 and HCC4011 cells, but not of low-AXL-expressing HCC827 cells. Besides, ONO-7475 enhances Osimertinib efficacy on the viability of cell lines PC-9, PC-9KGR, and HCC4011, and H1975, all of which express high levels of AXL. But it has a marginal effect on the viability of cell lines HCC827, HCC4006, and H3255 with low levels of AXL[1]. ONO-7475 (1 μM; 4 or 48 hours) combines with Osimertinib markedly inhibits the phosphorylation of AXL, AKT, and p70S6K compared with the treatment of the high-AXL-expressing cell lines treated with Osimertinib alone at 4 hours. It combines with osimertinib increases cleaved PARP in PC-9 and HCC4011 cells compared with the treatment with Osimertinib alone[1]. Cell Viability Assay[1] Cell Line: High AXL-expressing cell: PC-9,HCC4011,H1975, PC-9KGR cells Low AXL-expressing cell: HCC827, HCC4006, and H3255 cells Concentration: 0.0001 μM; 0.001 μM; 0.01 μM; 0.1 μM; 1 μM Incubation Time: 72 hours Result: Increased the sensitivity and efficacy to Osimertinib in AXL-high level cells. Western Blot Analysis[1] Cell Line: PC-9, HCC4011 cells Concentration: 1 μM Incubation Time: 4 or 48 hours Result: Increased p-AXL, AKT, and p70S6K expression at 4 hours and increases p-PARP expression at 48 hours when combindes with Osimertinib.
In Vivo	ONO-7475 (oral gavage; 10 mg/kg or combines with 5 mg/kg Osimertinib; 29 days) treatment alone has little effect on the tumor growth. Besides, Osimertinib alone causes tumor regression within one week, but the tumors reappear within three weeks. The combined initial treatment causes tumor regression and the size of tumors is maintained for 4 weeks. No apparent adverse events, including weight loss are observed during these treatments[1]. Animal Model: Mouse CDX model using high-AXL-expressing PC-9KGR cells (exon 19 deletion and the exon21-T790M mutation in EGFR)[1] Dosage: 10 mg/kg; once daily; 19 days Administration: oral gavage Result: Had little effects alone, but combined treatments significantly decreased tumor volume without reappear.
References	[1]. Okura N, et al. ONO-7475, a Novel AXL Inhibitor, Suppresses the Adaptive Resistance to Initial EGFR-TKI Treatment in EGFR-Mutated Non-Small Lung Cancer.Clin Cancer Res. 2020 Jan 17. [2]. Ruvolo PP, et al. Anexelekto/MER tyrosine kinase inhibitor ONO-7475 arrests growth and kills FMS-like tyrosine kinase 3-internal tandem duplication mutant acute myeloid leukemia cells by diverse mechanisms.Haematologica. 2017 Dec;102(12):2048-2057.

Molecular Formula	C₃₂H₂₆N₄O₆
Molecular Weight	562.57

Shanghai Nianxing Industrial Co., Ltd
China
Product Name: ONO-7475
Price: Negotiable
Purity: 98.0%
Delivery: 10 Day
Contact: Huang
Email: sales@echemcloud.com

ShangHai DC Chemicals Co.,Ltd
China
Product Name: ONO-7475
Price: ￥Inquiry/1g
Purity: 98.9%
Delivery: 1 Day
Contact: Tony Lai
Email: order@dcchemicals.com

DC Chemicals Limited
China
Product Name: ONO-7475
Price: $600.0/100mg $950.0/250mg $1900.0/1g
Purity: 98.0%
Delivery: 1 Day
Contact: Tony Cao
Email: sales@dcchemicals.com

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