<生产厂家价格>

荧光素

荧光素用途

Fluorescein是一种合成的荧光示踪剂。

荧光素名称

[ CAS 号 ]:
2321-07-5

[ 中文名 ]:
荧光素

[ 英文名 ]:
Fluorescein

[中文别名 ]:

[英文别名 ]:

D and C Yellow No. 7
D&C Yellow No. 7
D+C Yellow No. 7
Fluorescein (free acid)
3',6'-Fluorandiol
Fluorecein
Eosin
YELLOW 7
KI201
MFCD00005050

荧光素生物活性

[ 描述 ]:

Fluorescein是一种合成的荧光示踪剂。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

研究领域 >> 其他

[体外研究]

荧光素染料是常见的荧光探针。它在氩激光波长(488nm)下具有非常高的摩尔吸光系数,大的荧光量子产率和高光稳定性使其成为非常有用和灵敏的荧光标记。荧光素可以许多衍生物商购获得,例如异硫氰酸荧光素和荧光素琥珀酰亚胺酯,它们可以共价连接到大分子和氨基酸上。可以以非常高的灵敏度检测标记的分子,其用于例如毛细管电泳。荧光素的发射光谱广泛地重叠四甲基罗丹明的吸收光谱,四甲基罗丹明是一种相关的强荧光染料,使得该对非常适合于能量转移实验以确定标记的大分子内和之间的距离。水溶液中的荧光素以阳离子,中性,阴离子和双阴离子形式存在,使其吸收和荧光性质强烈依赖于pH [1]。

[参考文献]

[1]. R Sjöback, et al. Absorption and fluorescence properties of fluorescein. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

[相关活性小分子]

荧光素物理化学性质

[ 密度 ]:
1.6±0.1 g/cm3

[ 沸点 ]:
620.8±55.0 °C at 760 mmHg

[ 熔点 ]:
320 °C(lit.)

[ 分子式 ]:
C₂₀H₁₂O₅

[ 分子量 ]:
332.306

[ 闪点 ]:
232.6±25.0 °C

[ 精确质量 ]:
332.068481

[ PSA ]:
87.74000

[ LogP ]:
2.98

[ 外观性状 ]:
橙色-红色结晶粉末

[ 蒸汽压 ]:
0.0±1.9 mmHg at 25°C

[ 折射率 ]:
1.792

[ 储存条件 ]:
−20°C

[ 水溶解性 ]:
insoluble

荧光素MSDS

详细MSDS(安全技术说明书)

荧光素毒性和生态

CHEMICAL IDENTIFICATION

RTECS NUMBER :: LM5075000

CHEMICAL NAME :: Fluorescein

CAS REGISTRY NUMBER :: 2321-07-5

BEILSTEIN REFERENCE NO. :: 0094324

LAST UPDATED :: 199707

DATA ITEMS CITED :: 10

MOLECULAR FORMULA :: C20-H12-O5

MOLECULAR WEIGHT :: 332.32

WISWESSER LINE NOTATION :: T C666 BO EVJ IR BVQ& MQ

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 600 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 300 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Unreported

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 600 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rabbit

DOSE/DURATION :: 300 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Unreported

SPECIES OBSERVED :: Rodent - guinea pig

DOSE/DURATION :: 400 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

MUTATION DATA

TYPE OF TEST :: DNA adduct

TEST SYSTEM :: Bacteria - Escherichia coli

DOSE/DURATION :: 15 umol/L

REFERENCE :: MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 89,95,1981 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - 33350 No. of Facilities: 495 (estimated) No. of Industries: 18 No. of Occupations: 21 No. of Employees: 5188 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - 33350 No. of Facilities: 2171 (estimated) No. of Industries: 42 No. of Occupations: 55 No. of Employees: 88495 (estimated) No. of Female Employees: 21558 (estimated)

荧光素安全信息

[ 符号 ]:

GHS07

[ 信号词 ]:
Warning

[ 危害声明 ]:
H319

[ 警示性声明 ]:
P305 + P351 + P338

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危害码 (欧洲) ]:
Xi:Irritant;

[ 风险声明 (欧洲) ]:
R36;R43

[ 安全声明 (欧洲) ]:
S22-S24/25-S37/39-S26

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

[ RTECS号 ]:
LM5075000

[ 包装等级 ]:
I; II; III

[ 海关编码 ]:
3204200000

荧光素合成路线

详细合成路线

荧光素上下游产品

荧光素上游产品

荧光素下游产品

荧光素制备

1.将间苯二酚加热至150℃，使之全部熔融，边搅拌边加入理论量的邻苯二甲酸酐，混匀并熔融后升温至185℃，保温半小时，然后慢慢加入适量新焙烧的无水氯化锌，当完全溶解后，逐渐升温至210～215℃，整个过程均需不停地搅拌：

当反应液开始变稠时，停止搅拌，继续在此温度下加热至完全固化，研碎后得粉状粗品。将粉状粗品与稀盐酸混合加热煮沸，以浸出氯化锌和剩余的间苯二酚，抽滤后水洗、干燥，再用乙醇提取，晾干后即得红色固体荧光黄。

荧光素海关

[ 海关编码 ]: 2932999099

[ 中文概述 ]:
2932999099. 其他仅含氧杂原子的杂环化合物. 增值税率:17.0%. 退税率:13.0%. 监管条件:无. 最惠国关税:6.5%. 普通关税:20.0%

[ 申报要素 ]: 品名, 成分含量, 用途

[ Summary ]:
2932999099. other heterocyclic compounds with oxygen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

荧光素文献

CPEB1 modulates differentiation of glioma stem cells via downregulation of HES1 and SIRT1 expression.

Oncotarget 5(16) , 6756-69, (2014)

Glioma stemness has been recognized as the most important reason for glioma relapse and drug resistance. Differentiation of glioma stem cells (GSCs) has been implicated as a novel approach to target r...

Cell-cell adhesions and cell contractility are upregulated upon desmosome disruption.

PLoS ONE 9(7) , e101824, (2014)

Desmosomes are perturbed in a number of disease states - including genetic disorders, autoimmune and bacterial diseases. Here, we report unexpected changes in other cell-cell adhesion structures upon ...

Gene therapy with AAV2-CDNF provides functional benefits in a rat model of Parkinson's disease.

Brain Behav. 3(2) , 75-88, (2013)

Cerebral dopamine neurotrophic factor (CDNF) protein has been shown to protect the nigrostriatal dopaminergic pathway when given as intrastriatal infusions in rat and mouse models of Parkinson's disea...

更多文献

荧光素