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普鲁兰酶

更新时间:2024-01-02 19:06:21

普鲁兰酶结构式
普鲁兰酶结构式
品牌特惠专场
常用名 普鲁兰酶 英文名 PULLULANASE
CAS号 9075-68-7 分子量 N/A
密度 1.25 g/mL at 25 °C(lit.) 沸点 N/A
分子式 N/A 熔点 N/A
MSDS 中文版 美版 闪点 N/A

 普鲁兰酶用途


普鲁兰酶(R-酶)是一种淀粉脱支酶,常用于生化研究。普鲁兰酶水解淀粉、支链淀粉、普鲁兰和相关寡糖中的α-1,6葡萄糖苷键。普鲁兰酶在糖化过程中将多糖转化为小的发酵糖[1]。

 普鲁兰酶名称

中文名 普鲁兰酶
英文名 Pullulanase
中文别名 聚麦芽三糖酶 | 普鲁蓝酶 | 茁霉多糖酶 | 普鲁兰酶(2-8℃) | 支链淀粉酶(+4℃)
英文别名 更多

 普鲁兰酶生物活性

描述 普鲁兰酶(R-酶)是一种淀粉脱支酶,常用于生化研究。普鲁兰酶水解淀粉、支链淀粉、普鲁兰和相关寡糖中的α-1,6葡萄糖苷键。普鲁兰酶在糖化过程中将多糖转化为小的发酵糖[1]。
相关类别
参考文献

[1]. Hii SL, et al. Pullulanase: role in starch hydrolysis and potential industrial applications. Enzyme Res. 2012;2012:921362.  

 普鲁兰酶物理化学性质

密度 1.25 g/mL at 25 °C(lit.)
外观性状 lyophilized powder
储存条件

2 -8 °C 密闭、阴凉干燥处

稳定性

Other Notes View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Preparation Note Highly purified by a modification of ion exchange chromatography.

Unit Definition One unit will liberate 1.0 μmole of maltotriose (measured as glucose) from pullulan per min at pH 5.0 at 25 °C .

Physical form Suspension in 3.2 M (NH4)2SO4 solution, pH 6.2

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1. 性状:棕黄色细粉或近白色液体。

2. 密度(g/mL, 25 ℃ ):1.25

3. 相对蒸汽密度(g/mL,空气=1):未确定

4. 熔点(ºC):未确定

5. 沸点(ºC,常压):未确定

6. 沸点(ºC,5.2kPa):未确定

7. 折射率:未确定

8. 闪点(ºC):未确定

9. 比旋光度(º):未确定

10. 自燃点或引燃温度(ºC):未确定

11. 蒸气压(kPa,20ºC):未确定

12. 饱和蒸气压(kPa,60ºC):未确定

13. 燃烧热(KJ/mol):未确定

14. 临界温度(ºC):未确定

15. 临界压力(KPa):未确定

16. 油水(辛醇/水)分配系数的对数值:未确定

17. 爆炸上限(%,V/V):未确定

18. 爆炸下限(%,V/V):未确定

19. 溶解性:溶于水不溶于乙醇、氯仿和乙醚

 普鲁兰酶安全信息

个人防护装备 Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
安全声明 (欧洲) 22-24/25
危险品运输编码 NONH for all modes of transport
WGK德国 3

 普鲁兰酶制备

由产气克雷伯氏杆菌(Klebsiella aerogenes)在受控发酵条件下制备而成。

 普鲁兰酶文献49

更多文献
Gene cloning and enzymatic characterization of alkali-tolerant type I pullulanase from Exiguobacterium acetylicum.

Lett. Appl. Microbiol. 60(1) , 52-9, (2015)

A pullulanase gene (Pul3YH5) of 2568 bp, which encodes a protein containing 855 amino acid residues, was cloned from the alkaliphilic bacterium Exiguobacterium acetylicum YH5. The pullulanase (Pul3YH5...

Carrier free co-immobilization of alpha amylase, glucoamylase and pullulanase as combined cross-linked enzyme aggregates (combi-CLEAs): a tri-enzyme biocatalyst with one pot starch hydrolytic activity.

Bioresour. Technol. 147 , 269-75, (2013)

A tri-enzyme biocatalyst "combi-CLEAs" with starch hydrolytic activity was prepared from commercially available alpha amylase, glucoamylase and pullulanase preparations by aggregating enzymes with amm...

Properties of retrograded and acetylated starch produced via starch extrusion or starch hydrolysis with pullulanase.

Carbohydr. Polym. 97(2) , 551-7, (2013)

The aim of the present study was to determine the impact of serial modifications of starch, including firstly starch extrusion or hydrolysis with pullulanase, followed by retrogradation (through freez...

 普鲁兰酶英文别名

LIMIT DEXTRINASE
MFCD00081941
AMYLOPECTIN 6-GLUCONOHYDROLASE
EC 3.2.1.41
PULLULANASE FROM BACILLUS*ACIDOPULLULYTI
PULLULANASE FROM BACILLUS SP., POWDER, ~ 1 U/MG
pullulanase from klebsiella pneumoniae
pullulanase from bacillus sp.
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