2762302-75-8

2762302-75-8 structure

Name: DENV-IN-4
Chemical Name: DENV-IN-4
CAS Number: 2762302-75-8
Molecular Formula: C₂₈H₃₂N₄O₄Si
Molecular Weight: 516.66
Create Date: 2022-05-19 13:58:06
Modify Date: 2024-01-09 17:34:37
DENV-IN-4 is a potent DENV inhibitor (DENV EC50=4.79 μM, Vero CC50>100 μM, SI>20.9). DENV-IN-4 can inhibit the expression level of DENV2 with concentration-dependence and reduce RNA-dependent RNA polymerase (RdRp) enzymatic activity. DENV-IN-4 has antiviral effect[1].

Name	DENV-IN-4

Description	DENV-IN-4 is a potent DENV inhibitor (DENV EC50=4.79 μM, Vero CC50>100 μM, SI>20.9). DENV-IN-4 can inhibit the expression level of DENV2 with concentration-dependence and reduce RNA-dependent RNA polymerase (RdRp) enzymatic activity. DENV-IN-4 has antiviral effect[1].
Related Catalog	Research Areas >> Infection Signaling Pathways >> Cell Cycle/DNA Damage >> DNA/RNA Synthesis
Target	EC50=4.79 μM (DENV)[1]
In Vitro	DENV-IN-4 (compound 15) (0.064-200 μM; 3 days) has antiviral effect and not related to its toxicity (DENV EC50=4.79 μM, Vero CC50>100, SI>20.9)[1]. DENV-IN-4 (3.125-50 μM in Vero, 3.75-30 μM in A549 and Huh-7; 48 hours) inhibits the expression level of DENV2 with concentration-dependence, and inhibition curves were smoother in Vero[1]. DENV-IN-4 (7.5 and 15 μM; 48 hours) can effectively inhibit DENV infection in Vero cells, and completely inhibited at 15 μM[1]. DENV-IN-4 (0-15 μM; 48 hours) inhibits DENV2 E protein production with good concentration-dependent relationship in Vero cells[1]. Cell Cytotoxicity Assay Cell Line: Vero[1] Concentration: 0.064-200 μM Incubation Time: 3 days Result: Showed antiviral effect of DENV-IN-4 was not related to its toxicity (Vero EC50=4.79 μM, CC50>100, SI>20.9). RT-PCR Cell Line: Vero, A549 and Huh-7 cells[1] Concentration: 3.125-50 μM in Vero, 3.75-30 μM in A549 and Huh-7 Incubation Time: 48 hours Result: Inhibited the expression level of DENV2 with concentration-dependence, and inhibition curves were smoother in Vero. Western Blot Analysis Cell Line: Vero[1] Concentration: 7.5 and 15 μM Incubation Time: 48 hours Result: Effectively inhibited DENV infection and completely inhibited at 15 μM. Immunofluorescence Cell Line: Vero[1] Concentration: 0-15 μM Incubation Time: 48 hours Result: Inhibited DENV2 E protein production with good concentration-dependent relationship.
References	[1]. Qian W, et al. Design, synthesis, discovery and SAR of the fused tricyclic derivatives of indoline and imidazolidinone against DENV replication and infection [published online ahead of print, 2022 Jan 24]. Bioorg Chem. 2022;120:105639.

Molecular Formula	C₂₈H₃₂N₄O₄Si
Molecular Weight	516.66

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