In Vitro |
(-)-Hinesol (0-25 µg/ml; 24, 48 h) shows antiproliferative activity of the A549 and NCI-H1299 cells in a dose- and time-dependent manner[1]. (-)-Hinesol (0, 2, 8 µg/ml; 24 h) induces apoptosis and cell cycle arrest at G0/G1 phase with increases the expression of Bax and decreases the expression of Bcl-2 and cyclin D1[1]. (-)-Hinesol (0, 2, 8 µg/ml; 24 h) decreases the expression of phosphor-ERK1/2, phosphor-MEK1/2, phosphor-IκBα and -p65 level, and shows no change for the total protein levels of IκBα and p65 in A549 cells[1]. Cell Proliferation Assay[1] Cell Line: A549, NCI-H1299 cells Concentration: 0-25 µg/ml Incubation Time: 24, 48 h Result: Inhibited the proliferation of the A549 and NCI-H1299 cells in a dose- and time-dependent manner. Apoptosis Analysis[1] Cell Line: A549 cells Concentration: 0, 2, 8 µg/ml Incubation Time: 24 h Result: Induced apoptosis with the apoptotic cells was increased to 21.2 ± 0.96% and 36 ± 1.04% after treatment with hinesol at 2 and 8 μg/mL, respectively. Western Blot Analysis[1] Cell Line: A549 cells Concentration: 0, 2, 8 µg/ml Incubation Time: 24 h Result: Increased the protein expression of Bax and decreased the expression of Bcl-2. Cell Cycle Analysis[1] Cell Line: A549 cells Concentration: 0, 2, 8 µg/ml Incubation Time: 24 h Result: Showed concentration-dependent increase in the percentage of cell in the G0/G1 phase, and a decrease of the percentage in G2/M phase.
|