821794-90-5
821794-90-5 structure
- Name: CMK
- Chemical Name: 1-[4-amino-7-(3-hydroxypropyl)-5-(4-methylphenyl)pyrrolo[2,3-d]pyrimidin-6-yl]-2-chloroethanone
- CAS Number: 821794-90-5
- Molecular Formula: C18H19ClN4O2
- Molecular Weight: 358.822
- Catalog: Signaling Pathways MAPK/ERK Pathway Ribosomal S6 Kinase (RSK)
- Create Date: 2018-12-30 20:09:38
- Modify Date: 2024-01-02 20:07:53
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CMK is a RSK2 kinase inhibitor which exhibits similar potency but less chemical stability compared with FMK.
| Name | 1-[4-amino-7-(3-hydroxypropyl)-5-(4-methylphenyl)pyrrolo[2,3-d]pyrimidin-6-yl]-2-chloroethanone |
|---|---|
| Synonyms |
Ethanone, 1-[4-amino-7-(3-hydroxypropyl)-5-(4-methylphenyl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl]-2-chloro-
CS-0404 1-[4-Amino-7-(3-hydroxypropyl)-5-(4-methylphenyl)-7H-pyrrolo[2,3-d]pyrimidin-6-yl]-2-chloroethanone CMK| 1-(4-Amino-7-(3-hydroxypropyl)-5-(p-tolyl)-7h-pyrrolo[2,3-d]pyrimidin-6-yl)-2-chloroethanone CMK |
| Description | CMK is a RSK2 kinase inhibitor which exhibits similar potency but less chemical stability compared with FMK. |
|---|---|
| Related Catalog | |
| In Vitro | CMK inhibits the growth of Cdc5 (L158G) with IC50 of 36 nM, greater than 30 μM for wild type Cdc5. CMK exhibits a concentration-dependent first cell cycle mitotic arrest in the cdc5-as1 strain with an IC50 of 1.1 μM. CMK inhibition of Cdc5 (L158G) leads to a first cell cycle anaphase arrest and delay in anaphase spindle migration[1]. |
| Kinase Assay | For Cdc5 kinase assays, Spc72-TAP is isolated from cell extract by pulldown with IgG sepharose or rabbit IgG coupled to M-270 epoxy dynabeads and incubated in kinase buffer (25 mM HEPES, pH 8.0, 60 mM KCl, 15 mM MnCl2, 100 μg/mL BSA, 80 nM microcystin, 1mM DTT, 100 μM 200 μCi/mL [γ-32P]ATP) in the absence or presence of 100 ng purified baculovirus expressed 6xHis-Cdc5. 32P incorporation is visualized on a Typhoon PhosphorImager, and images are processed using ImageQuant software. |
| References |
| Density | 1.4±0.1 g/cm3 |
|---|---|
| Boiling Point | 647.5±55.0 °C at 760 mmHg |
| Molecular Formula | C18H19ClN4O2 |
| Molecular Weight | 358.822 |
| Flash Point | 345.4±31.5 °C |
| Exact Mass | 358.119659 |
| PSA | 94.03000 |
| LogP | 1.55 |
| Vapour Pressure | 0.0±2.0 mmHg at 25°C |
| Index of Refraction | 1.670 |
| Storage condition | 2-8℃ |