874911-96-3

874911-96-3 structure

Name: ZK 756326
Chemical Name: 2-[2-[4-[(3-phenoxyphenyl)methyl]-1-piperazinyl]ethoxy]ethanoldihydrochloride
CAS Number: 874911-96-3
Molecular Formula: C₂₁H₂₈N₂O₃
Molecular Weight: 356.46
Catalog: Signaling Pathways GPCR/G Protein CCR
Create Date: 2017-11-10 06:33:24
Modify Date: 2024-01-03 06:12:38
ZK756326 is a nonpeptide chemokine receptor agonist for the CC chemokine receptor CCR8.

Name	2-[2-[4-[(3-phenoxyphenyl)methyl]-1-piperazinyl]ethoxy]ethanoldihydrochloride
Synonyms	2-[2-[4-[(3-Phenoxyphenyl)methyl]-1-piperazinyl]ethoxy]ethanol ZK 756326 MFCD09038571

Description	ZK756326 is a nonpeptide chemokine receptor agonist for the CC chemokine receptor CCR8.
Related Catalog	Signaling Pathways >> GPCR/G Protein >> CCR Research Areas >> Cancer Research Areas >> Endocrinology Signaling Pathways >> Immunology/Inflammation >> CCR
Target	IC50: 1.8 μM (CCR8)[1]
Cell Assay	U87 MG cells expressing CCR8 are plated on poly-D-lysine-coated black 96-well plates at 10,000 cells/well and are cultured overnight. Cells are then loaded with Calcium 3, a Ca2+-sensitive non-wash fluorescence dye, for 60 min at 37°C in Hanks' balanced salts solution containing 20 mM HEPES, 3.2 mM CaCl2, 1% (v/v) fetal bovine serum, and 2.5 mM probenecid. Changes in intracellular free-Ca2+ concentration are measured with Fluorometric Imaging Plate Reader (FLIPR 3) immediately after the addition of agonist at room temperature. Cross-desensitization experiments are performed by a first addition of the agonist (CCL1 at 30 nM or ZK 756326 at 3 μM), immediately followed by a second addition of 100 nM CCL1[1].
References	[1]. Haskell CA, et al. Identification and characterization of a potent, selective nonpeptide agonist of the CC chemokine receptor CCR8. Mol Pharmacol. 2006 Jan;69(1):309-16.