Calmodulin-Dependent Protein Kinase II (290-309)

Modify Date: 2024-01-11 19:02:52

Calmodulin-Dependent Protein Kinase II (290-309) structure	Common Name	Calmodulin-Dependent Protein Kinase II (290-309)
	CAS Number	115044-69-4	Molecular Weight	2273.83000
	Density	N/A	Boiling Point	N/A
	Molecular Formula	C₁₀₃H₁₈₅N₃₁O₂₄S	Melting Point	N/A
	MSDS	USA	Flash Point	N/A

Use of Calmodulin-Dependent Protein Kinase II (290-309)

Calmodulin-Dependent Protein Kinase II (290-309) is a potent CaMK antagonist with an IC50 of 52 nM for inhibition of Ca2+/calmodulin-dependent protein kinase II.

Name	Calmodulin-Dependent Protein Kinase II (290-309)
Synonym	More Synonyms

Description	Calmodulin-Dependent Protein Kinase II (290-309) is a potent CaMK antagonist with an IC50 of 52 nM for inhibition of Ca2+/calmodulin-dependent protein kinase II.
Related Catalog	Signaling Pathways >> Neuronal Signaling >> CaMK Research Areas >> Neurological Disease Peptides
Target	IC50: 52 nM (calmodulin-dependent protein kinase II)[1]
In Vitro	Peptide 290 to 309 is found to be a potent calmodulin antagonist with an IC50 of 52 nM for inhibition of Ca2+/calmodulin-dependent protein kinase. Neither truncation from the amino terminus (peptide 296-309) nor extension in the carboxyl-terminal direction (peptide 294-319) markedly affects calmodulin binding, whereas shortening the peptide from the carboxyl terminus (peptide 290-302) or from both ends (peptide 295-304) results in the elimination of this activity[1].
Kinase Assay	Ca2+/CaM-dependent phosphodiesterase is assayed at 30°C. The reaction mixture contains 40 mM Tris-HCl, pH 8.0, 5 mM magnesium acetate, 1 mM calcium chloride, 30 μM cGMP, 0.15 pCi of [3H]cGMP, 1 mM dithiothreitol, 20% glycerol, 0.64 mg/mL bovine serum albumin, 2.38 nM CaM, 50 pM CaM-deficient phosphodiesterase, and various concentrations of Calmodulin-Dependent Protein Kinase II (290-309). Following preincubation of the reaction mixture at 30°C, the reaction is initiated by the addition of substrate. After 50 min the reaction is terminated by boiling. Conditions are selected in which the reactions are linear with respect to time[1].
References	[1]. Payne ME, et al. Calcium/calmodulin-dependent protein kinase II. Characterization of distinct calmodulin binding and inhibitory domains. J Biol Chem. 1988 May 25;263(15):7190-5.

Molecular Formula	C₁₀₃H₁₈₅N₃₁O₂₄S
Molecular Weight	2273.83000
Exact Mass	2272.39000
PSA	952.95000
LogP	7.21720
Storage condition	2-8℃

MSDS(Chinese)

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Safety Phrases	22-24/25
RIDADR	NONH for all modes of transport
WGK Germany	3

Long-term potentiation is associated with an increased activity of Ca2+/calmodulin-dependent protein kinase II.

J. Biol. Chem. 268 , 7863, (1993)

Among the molecular mechanisms that have been proposed to contribute to long-term potentiation in hippocampus are the activation and autophosphorylation of Ca2+/calmodulin-dependent protein kinase II ...

Activation of calcium channels by cAMP in STC-1 cells is dependent upon Ca2+ calmodulin-dependent protein kinase II.

Biochem. Biophys. Res. Commun. 254 , 699, (1999)

Activation of L-type calcium channels in the neuroendocrine, cholecytstokinin-secreting cell line, STC-1, is vital for secretion of CCK. In the present study, the regulation of L-type Ca2+ channels by...

CAM KINASE II (290-309)

CALMODULIN-DEPENDENT PROTEIN KINASE II (290-309)

CaM kinase II (290-309)

CALMODULIN-DEPENDENT PROTEIN KINASE II FRAGMENT 290-309

LKKFNARRKLKGAILTTMLA

CALMODULIN DEPENDENT PROTEIN KINASE II

CALMODULIN BINDING DOMAIN

LEU-LYS-LYS-PHE-ASN-ALA-ARG-ARG-LYS-LEU-LYS-GLY-ALA-ILE-LEU-THR-THR-MET-LEU-ALA

CA2+/CALMODULIN-DEPENDENT PROTEIN KINASE II (290-309)

CALMODULIN-DEPENDENT PROTEIN KINASE IIFR AGMENT 290

H-LEU-LYS-LYS-PHE-ASN-ALA-ARG-ARG-LYS-LEU-LYS-GLY-ALA-ILE-LEU-THR-THR-MET-LEU-ALA-OH